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Fig. 27 | Proteome Science

Fig. 27

From: Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs)

Fig. 27

(a) Schematic representation of the procedure for PAL of lectins with carbohydrate photoprobe and isolation of photo-crosslinked proteins via tandem application of SPAAC, biotin-streptavidin enrichment and a photo-release step. (b) Design of the multivalent photo-affinity glycoprobe (1) and the photo-cleavable biotin affinity tag (2). Figure reproduced from ref. [79] with permission from ScienceDirect publication

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ISSN: 1477-5956